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Peptide Interactions intermediate

FLAG-tag → Anti-FLAG (Affinity Purification)

Comprehensive reference for FLAG-tag → Anti-FLAG (Affinity Purification), a peptide compound with applications in research and therapeutics.

By Encyclopeptide Editorial | 1 min read
peptide-interactions peptide oligopeptide

Overview

FLAG-tag → Anti-FLAG (Affinity Purification) is a bioactive peptide with well-characterized properties and therapeutic applications.

Mechanism of Action

FLAG peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) recognized by anti-FLAG monoclonal antibodies through complementary determining regions. M1 antibody requires Ca²⁺ for binding (binds N-terminal Asp-Tyr). M2 antibody recognizes internal epitope (Ca²⁺-independent). Elution with FLAG peptide (100-200 μg/mL) or EDTA (M1).

References

  • Wikipept Peptide Database
  • Primary literature (see individual entries)

Chemical Identity

PropertyValue
NameFLAG-tag → Anti-FLAG (Affinity Purification)
CategoryPeptide Interactions

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