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Immunomodulatory Peptides intermediate

Interferon Alfa

Type I interferon glycoprotein with antiviral, antiproliferative, and immunomodulatory effects for hepatitis and cancer.

By Encyclopeptide Editorial | 2 min read
interferon antiviral immunomodulator hepatitis

Chemical Identity

PropertyValue
Molecular Weight~19 kDa
Amino Acids165-166 (varies by subtype)
Gene FamilyIFNA (13 subtypes)
ReceptorIFNAR1/IFNAR2
RouteSC, IM

Structure

Interferon alfa (IFN-alpha) is a family of type I interferons consisting of 13 subtypes (IFN-alpha1, 2a, 2b, etc.) with 165-166 amino acids and two disulfide bonds. All subtypes share the same receptor (IFNAR1/IFNAR2) and have similar biological activities, though potencies differ.

Mechanism of Action

IFN-alpha binds IFNAR1/IFNAR2, activating JAK1/TYK2 and STAT1/STAT2 signaling. This induces hundreds of interferon-stimulated genes (ISGs) that establish an antiviral state (PKR, OAS, Mx proteins), enhance MHC class I expression, activate NK cells and cytotoxic T cells, and inhibit cell proliferation.

Clinical Applications

  • Hepatitis B: Pegylated IFN-alpha (peginterferon alfa)
  • Hepatitis C: With ribavirin (now replaced by DAAs)
  • Hairy cell leukemia: First-line therapy
  • Melanoma: Adjuvant high-dose therapy
  • Kaposi sarcoma: AIDS-related KS

Pharmacokinetics

  • Half-life: 2-4 hours (native), 40-80 hours (pegylated)
  • Tmax: 4-8 hours (SC)
  • Bioavailability: 80% (SC)
  • Metabolism: Renal and hepatic
  • Route: SC, IM (pegylated: SC weekly)

Safety and Side Effects

Flu-like symptoms (90%), fatigue (70%), depression (30%), neutropenia, thrombocytopenia, autoimmune thyroiditis, and hepatotoxicity. Dose-limiting toxicity for many patients.

References

  • Isaacs, A., & Lindenmann, J. (1957). Virus interference: the interferon. Proceedings of the Royal Society B, 147, 258-267.
  • Lindsay, K.L. (1997). Therapy of hepatitis C: overview. Hepatology, 26, 71S-77S.

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