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Therapeutic Peptides intermediate

Teriparatide

Teriparatide is a recombinant PTH(1-34) fragment used to stimulate osteoblast activity and new bone formation in severe osteoporosis, the first anabolic skeletal agent.

By Encyclopeptide Editorial | 2 min read
PTH osteoporosis anabolic bone-formation parathyroid

Chemical Identity

PropertyValue
Chemical FormulaC181H291N55O51S
Molecular Weight4117.72 g/mol
CAS Number52232-67-4
IUPAC NameHuman PTH(1-34) fragment
Peptide ClassParathyroid Hormone Analog
Sequence HomologyIdentical to human PTH(1-34)

Structure

Teriparatide is the recombinant human parathyroid hormone (PTH) fragment comprising amino acids 1-34 of the full-length 84-amino acid hormone. This N-terminal fragment retains full biological activity at the PTH/PTHrP receptor. The peptide is produced by recombinant DNA technology in Escherichia coli.

Mechanism of Action

Teriparatide acts as an anabolic agent for bone when administered intermittently (daily injection). It binds to PTH1 receptors on osteoblasts and osteocytes, stimulating:

  • Osteoblast proliferation and differentiation
  • RANKL expression (paradoxically increasing bone resorption transiently)
  • Net bone formation exceeding resorption with daily dosing
  • Wnt/β-catenin signaling pathway activation

This intermittent PTH exposure produces a net anabolic effect, contrasting with the catabolic effect of continuous PTH elevation in hyperparathyroidism.

Clinical Applications

Teriparatide (Forteo) is approved for:

  • Severe osteoporosis: Postmenopausal women and men at high fracture risk
  • Glucocorticoid-induced osteoporosis: In patients at high risk for fracture
  • Treatment failure: Patients who have failed or are intolerant to other osteoporosis therapies

In the pivotal trial, teriparatide reduced vertebral fractures by 65% and non-vertebral fractures by 53% over 18 months.

Pharmacokinetics

  • Half-life: 1 hour (subcutaneous)
  • Tmax: 30 minutes after subcutaneous injection
  • Bioavailability: 95%
  • Metabolism: Hepatic and renal proteolysis
  • Duration of therapy: Limited to 24 months due to osteosarcoma risk in preclinical studies

Safety and Side Effects

Common adverse effects include orthostatic hypotension (especially after first dose), dizziness, nausea, leg cramps, and hypercalcemia. Contraindicated in patients at increased risk for osteosarcoma (Paget disease, prior radiation therapy, open epiphyses). The 24-month treatment limit was established based on osteosarcoma findings in rats, though this has not been confirmed in humans.

References

  • Neer, R.M., et al. (2001). Effect of parathyroid hormone (1-34) on fractures and bone mineral density in postmenopausal women with osteoporosis. New England Journal of Medicine, 344, 1434-1441.
  • Saag, K.G., et al. (2007). Teriparatide or alendronate in glucocorticoid-induced osteoporosis. New England Journal of Medicine, 357, 2028-2039.

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