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Antimicrobial Peptides intermediate

Vancomycin

Vancomycin is a glycopeptide antibiotic from Amycolatopsis orientalis that inhibits cell wall synthesis in gram-positive bacteria, including MRSA.

By Encyclopeptide Editorial | 2 min read
glycopeptide antibiotic MRSA cell-wall anti-infective

Chemical Identity

PropertyValue
Chemical FormulaC66H75Cl2N9O24
Molecular Weight1449.25 g/mol
CAS Number1404-90-6
IUPAC NameVancomycin hydrochloride
Peptide ClassGlycopeptide Antibiotic
OriginAmycolatopsis orientalis

Structure

Vancomycin is a tricyclic glycopeptide consisting of seven amino acids, including several unusual non-proteinogenic residues (beta-hydroxytyrosine, 4-hydroxyphenylglycine, and beta-hydroxychloroethylglycine). Two chlorinated aromatic rings and three fused macrocyclic rings create a rigid cup-shaped structure. A disaccharide (vancosamine-glucose) is attached to ring 4. The heptapeptide core is produced non-ribosomally by NRPS enzymes.

Mechanism of Action

Vancomycin binds with high affinity to the D-alanyl-D-alanine (D-Ala-D-Ala) terminus of the peptidoglycan precursor lipid II, blocking both transglycosylation and transpeptidation steps of cell wall synthesis. This binding is stoichiometric and irreversible, making vancomycin bactericidal against most gram-positive organisms. Unlike beta-lactams, the target is the substrate rather than an enzyme.

Clinical Applications

Vancomycin is approved for:

  • MRSA infections: Bacteremia, endocarditis, pneumonia, osteomyelitis
  • Clostridioides difficile infection: Oral vancomycin for C. difficile colitis
  • Prosthetic joint infections: As part of combination therapy
  • Surgical prophylaxis: In patients with MRSA risk factors

Intravenous dosing requires therapeutic drug monitoring (trough 10-20 mcg/mL for serious infections). AUC/MIC ratio >400 is the preferred pharmacodynamic target.

Pharmacokinetics

  • Half-life: 4-6 hours (normal renal function); extended in renal impairment
  • Protein binding: 30-55%
  • Metabolism: Not significantly metabolized
  • Elimination: Primarily renal (80-90% unchanged)
  • Dialysis: Not significantly removed by hemodialysis

Safety and Side Effects

Red man syndrome (histamine-mediated flushing and hypotension with rapid infusion), nephrotoxicity (especially with concurrent nephrotoxins or trough >20 mcg/mL), ototoxicity (rare with modern dosing), and thrombocytopenia (vancomycin-dependent antibodies). Infusion rate should not exceed 10 mg/min to prevent red man syndrome.

References

  • Levine, D.P. (2006). Vancomycin: a history. Clinical Infectious Diseases, 42, S5-S12.
  • Rybak, M., et al. (2009). Therapeutic monitoring of vancomycin in adult patients. American Journal of Health-System Pharmacy, 66, 82-98.

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