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Transplant Peptides intermediate

Daclizumab

Humanized anti-CD25 antibody for transplant rejection prevention and multiple sclerosis (withdrawn due to safety concerns).

By Encyclopeptide Editorial | 2 min read
anti-CD25 IL-2-receptor transplant multiple-sclerosis

Chemical Identity

PropertyValue
Molecular Weight~144 kDa
StructureHumanized IgG1 monoclonal antibody
TargetCD25 (IL-2R alpha chain)
CAS Number152923-56-3
RouteIV (transplant), SC (MS)

Structure

Daclizumab (Zenapax, Zinbryta) is a humanized IgG1 monoclonal antibody targeting CD25. The complementarity-determining regions (CDRs) from a murine anti-CD25 antibody were grafted onto a human IgG1 framework, reducing immunogenicity while maintaining binding affinity.

Mechanism of Action

Daclizumab binds CD25 on activated T cells, blocking IL-2 binding and preventing IL-2-mediated T-cell proliferation. In MS, it may also expand regulatory CD56bright NK cells that suppress autoimmune T cells. It selectively targets activated immune cells without broad immunosuppression.

Clinical Applications

  • Kidney transplant: Induction therapy (Zenapax, discontinued)
  • Multiple sclerosis: Relapsing MS (Zinbryta, withdrawn 2018)
  • Uveitis: Investigational
  • Type 1 diabetes: Investigational (preserving beta-cell function)
  • Withdrawn: Due to immune-mediated encephalitis and hepatitis

Pharmacokinetics

  • Half-life: 20 days
  • Tmax: End of infusion
  • CD25 saturation: >90% for 120 days
  • Dosing: 1 mg/kg IV every 2 weeks (transplant)
  • Route: IV, SC

Safety and Side Effects

Infections, immune-mediated hepatitis, encephalitis (fatal cases led to withdrawal of Zinbryta), skin reactions, and thrombocytopenia. Zinbryta withdrawn from market in 2018 due to inflammatory brain and liver adverse events.

References

  • Vincenti, F., et al. (1998). Daclizumab for kidney transplant. New England Journal of Medicine, 338, 161-165.
  • Kappos, L., et al. (2016). Daclizumab for MS (DECIDE). New England Journal of Medicine, 375, 2200-2211.

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